ALDAGEN - Key Scientific References

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ALDH Activity as a Universal Marker of Active Stem/Progenitor Cells

	Laird DJ, De Tomaso AW, Weissman IL. Stem cells are units of natural selection in a colonial ascidian. Cell . 2005 Dec 29; 123:1351-1360.

	Mitchell JB, McIntosh K, Zvonic S, et al. The immunophenotype of human adipose derived cells: Temporal changes in stromal- and stem cell-associated markers. Stem Cells. 2005 Dec 1; [Epub ahead of print]

	Corti S, Locatelli F, Papadimitriou D, et al. Identification of a primitive brain-derived neural stem cell population based on aldehyde dehydrogenase activity. Stem Cells. 2005 Nov 17; [Epub ahead of print]

	David A. Hess, Louisa Wirthlin, Timothy C. Craft, et al. Transplanted Human Aldehyde Dehydrogenase Expressing Stem Cells Circulate and Engraft Nonhematopoietic Tissues. American Society of Hematology (ASH). 2005;1693.

	Cai J, Weiss ML, Rao MS. In search of "stemness". Experimental Hematology. 2004;32:585-598.

ALDESORT Captures Mesenchymal and Endothelial Progenitors Potentially Useful in Vascular Biology & Cardiology

	Benjamin J. Capoccia, Louisa Wirthlin, Rebecca Shepherd, et al. Bone Marrow-Derived Aldehyde Dehydrogenase Expressing Cells Possess Endothelial Progenitor Function in Addition to Hematopoietic Repopulating Ability and Aid in Blood Flow Recovery after Acute Ischemic Injury. American Society of Hematology (ASH). 2005;2663.

	David A. Hess, Louisa Wirthlin, Timothy C. Craft, et al. Transplanted Human Aldehyde Dehydrogenase Expressing Stem Cells Circulate and Engraft Nonhematopoietic Tissues. American Society of Hematology (ASH). 2005;1693.

	Mitchell JB, McIntosh K, Zvonic S, et al. The immunophenotype of human adipose derived cells: Temporal changes in stromal- and stem cell-associated markers. Stem Cells. 2005 Dec 1; [Epub ahead of print]

	Zhu S, Dong C, Gentry T, Balber A, Goldschmidt-Clermont PJ. Aldehyde Dehydrogenase-Positive Stem Cells From the Human Bone Marrow Differentiate Into Endothelial Cells More Efficiently Than CD133+/CD34+ Endothelial Progenitor Cells. American College of Cardiology (ACC). 2005.

	Gentry T, Winstead L, Foster SJ, Balber AE. Bone Marrow Cells Expressing High Levels of Aldehyde Dehydrogenase (ALDH) Exhibit Characteristics of Mesenchymal and Endothelial Progenitor Cells. Mesenchymal and Nonhematopoietic Stem Cell (MNSC). 2004;49.

	Gentry T, Winstead L, Deibert E, et al. Aldehyde Dehydrogenase (ALDH) and Surface Antigen Expression Define Hematopoietic Stem and Progenitor Cell (HSPC) Subsets Differentially Represented in Mobilized Peripheral Blood (PBSC), Umbilical Cord Blood (UCB), and Bone Marrow (BM). Tandem American Society for Blood and Marrow Transplantation and the International Bone Marrow Transplant Registry (ASBMT/IBMTR). 2004;63.

	Fiordalisi M, Deibert E, Winstead L, et al. Surface Phenotype of Bone Marrow Cells that Express High Levels of Aldehyde Dehydrogenase (ALDH). American Society of Hematology (ASH). 2003;4302.

ALDESORT Captures Highly Functional Human Hematopoietic Stem/Progenitor Cells for Clinical Applications

	Hess DA, Wirthlin L, Craft TP, et al. Selection based on CD133 and high aldehyde dehydrogenase activity isolates long-term reconstituting human hematopoietic stem cells. Blood, 2005 Nov 3, [Epub ahead of print]

	Martin Hildebrandt, Markus Schuler, Kirstin Rautenberg, Christian Gerecke, Wolf-Dieter Ludwig. Aldehyde Dehydrogenase- Positive Hematopoetic Progenitor Cells in Peripheral Blood and Progenitor Cell Apheresis Products: Characterization and Correlation with Kinetics of Engraftment. American Society of Hematology (ASH). 2005;1076.

	Hess DA, Wirthlin L, Craft TP, et al. Selection based on CD133 and high aldehyde dehydrogenase activity isolates long-term reconstituting human hematopoietic stem cells. Blood. 2005;06:2284.

	Storms RW, Green PD, Safford KM, et al. Distinct hematopoietic progenitor compartments are delineated by the expression of aldehyde dehydrogenase and CD34. Blood. 2005; 6:95-102.

	Lioznov MV, Freiberger P, Kröger N, Zander AR, Fehse B. Aldehyde dehydrogenase activity as a marker for the quality of hematopoietic stem cell transplants. Bone Marrow Transplantation. 2005;35:909-914.

	Hess DA, Wirthlin L, Craft TP, Herrbrich PE, Nolta JA. Purification of Cells with High Aldehyde Dehydrogenase Activity Reveals a CD133 Negative Population with Enhanced Repopulating Ability after Ex Vivo Culture. American Society of Gene Therapy (ASGT). 2005;1031.

	Hildebrandt M, Schuler M, Rautenberg K, Bargon R, Held T, Ludwig WD. Aldehyde Dehydrogenase-Positive Haematopoietic Progenitor Cells in Peripheral Blood and Apheresis Product: Characterisation and Correlation with Kinetics of Engraftment. European Blood and Marrow Transplantation. 2005

	Ute Meissauer, Joerg Hoffmann, Christian Scharenberg, Andreas Neubauer, Cornelia Brendel. Aldehyde Dehydrogenase Expression in Primitive Human Hematopoietic Progenitor Cells with Side Population Characteristic and in Samples from Patients with Acute Myeloid Leukemia. American Society of Hematology (ASH). 2005;4570.

	Cesare Perotti, Gianluca Viarengo, Claudia Del Fante, et al. Aldehyde Dehydrogenase (ALDH) Activity in Fresh (Pre-Freezing) and Post-Thawing Leukapheresis and Cord Blood Collections. American Society of Hematology (ASH). 2005;5276.

	Gentry T, Balber AE, Baucom C, et al. Efficient Enrichment and Flow Sorting of Aldehyde Dehydrogenase Bright Hematopoietic Progenitor Cells from Thawed, Banked Umbilical Cord Blood. Tandem American Society for Blood and Marrow Transplantation and the Center for International Blood and Marrow Transplant Research (ASBMT/CIBMTR). 2005;204.

	Hess DA, Meyerrose TE, Wirthlin L, et al. Functional characterization of highly purified human hematopoietic repopulating cells isolated based on aldehyde dehydrogenase activity. Blood. 2004;104:1648-655.

	Hess DA, Wirthlin L, Craft TP, Meyerrose TE, Nolta JA. CD133- Expressing Cells with High Aldehyde Dehydrogenase Activity Represent a Primitive Human Hematopoietic Stem Cell Population with Enhanced Repopulating Potential. American Society of Hematology (ASH). b2004;250.

	Christ O, Smith C, Leung K, Hamilton M, Eaves CJ. Identification of a New Population of Human Cord Blood Cells with Lymphoid-Restricted Reconstituting Potential in Sublethally Irradiated Immunodeficient Mice. American Society of Hematology (ASH). 2004;3213.

	Hess AD, Herrbrich PE, Wirthlin L, Craft TP, Nolta JA. Isolation of Human CD34- Cells with High Aldehyde Dehydrogenase Activity Reveals a Novel Population with Hematopoietic Repopulating Potential. American Society of Hematology (ASH). 2004;3214.

	Hess DA, Craft TP, Wirthlin L, Meyerrose TE, Herrbrich PE, Nolta JA. CD133 Expressing Cells with High Aldehyde Dehydrogenase Activity Represent a Novel Human Hematopoietic Stem Cell Population. International Society for Experimental Hematology (ISEH). 2004;273.

	Gentry T, Winstead L, Deibert E, et al. Aldehyde Dehydrogenase (ALDH) and Surface Antigen Expression Define Hematopoietic Stem and Progenitor Cell (HSPC) Subsets Differentially Represented in Mobilized Peripheral Blood (PBSC), Umbilical Cord Blood (UCB), and Bone Marrow (BM). Tandem American Society for Blood and Marrow Transplantation and the International Bone Marrow Transplant Registry (ASBMT/IBMTR). 2004;63.

	Fallon P, Gentry T, Balber AE, et al. Mobilized peripheral blood SSCloALDHbr cells have the phenotypic and functional properties of primitive haematopoietic cells and their number correlates with engraftment following autologous transplantation. British Journal of Haematology. 2003;122:99-108.

	Christ O, Hamilton MJ, Smith C, Eaves CJ. Short and Long Term Repopulating Cells in Human Cord Blood Display Different Levels of Aldehyde Dehydrogenase Activity as Revealed by Assays of BODIPY-Stained Cells in NOD/SCID Mice. American Society of Hematology (ASH). 2003;1178.

	Storms RW, Trujillo AP, Springer JB, et al. Isolation of primitive human hematopoietic progenitors on the basis of aldehyde dehydrogenase activity. Proc. Natl. Acad. Sci. USA.1999;96:9118-9123.

ALDESORT Captures Cells Potentially Useful in Neurological and Other Chronic Disease

	Corti S, Locatelli F, Papadimitriou D, et al. Transplanted ALDHhiSSClo Neural Stem Cells Generate Motor Neurons and Delay Disease Progression of nmd Mice, an Animal Model of SMARD1. Hum Mol Genet. 2005 Dec 8; [Epub ahead of print]

	Corti S, Locatelli F, Papadimitriou D, et al. Identification of a primitive brain-derived neural stem cell population based on aldehyde dehydrogenase activity. Stem Cells. 2005 Nov 17; [Epub ahead of print]

	D.A. Hess, L. W., T.P. Craft, P.E. Herrbrich, M.Roberts, M.H. Creer, M.S. Sands, J. A. Nolta. Transplanted human aldehyde dehydrogenase expressing stem cells circulate and highly engraft non-hematopoietic tissues. ASH. 2005.

	Hess DA, Craft TP, Wirthlin L, et al. Transplantation of Human Aldehyde Dehydrogenase Expressing Cell Leads to Widespread Tissue Distribution of Donor Cells in the Pancreas and Liver of NOD/SCID/MPSVII Mice. American Society of Gene Therapy (ASGT). 2005;460.

	Gentry T, Ford D, Balber A, Trisler D. Human Aldehyde Dehydrogenase Bright Bone Marrow Cells: Expression of Neural Markers and Growth Characteristics in a Culture System that Supports Expansion of Murine Bone Marrow Neural Progenitors. International Society for Cellular Therapy (ISCT). 2005;159.

	Giver CR, Foster SJ, Martin WD, Balber AE, Waller N. Injection of Blastocysts from Immunocompetent Mice with Human HPC having High ALDH Activity Results in Chimeric Animals with Low-Level Human Contribution in Hematopoietic and Non-Hematopoietic Tissues. American Society of Hematology. 2004;3224.

	Cai J, Cheng A, Luo Y, Mattson MP, Rao MS, Furukawa K. Membrane properties of rat embryonic multipotent neural stem cells. Journal of Neurochemistry. 2004;88:212-226.

	Giver CR, Foster SJ, Martin WD, Balber AE, Waller N. Injection of Blastocysts from Immunocompetent Mice with Human HPC having High ALDH Activity Results in Chimeric Animals with Low-Level Human Contribution in Hematopoietic and Non-Hematopoietic Tissues. American Society of Hematology (ASH). 2004;3224.

	Hess DA, Craft TP, Wirthlin L, et al. Transplantation of Human Aldehyde Dehydrogenase Expressing Cells Leads to Widespread Tissue Distribution of Donor Cells in the Novel NOD/SCID/MPSVII Xenotransplantation Model. American Society of Hematology (ASH). 2004;3601.

	Desjarlais T, Sanberg PR, Willing AE, et al. Human Umbilical Cord Blood Cells in Primary and Secondary Lymphoid Organs in a Mouse Model of Amyotrophic Lateral Sclerosis after Cerebral Intraventricular Transplantation. American Society for Neural Transplantation and Repair (ASNTR). 2004.

	Garbuzova-Davis SN, Willing AE, Saporta S, et al. Human Umbilical Cord Blood Cells as a Potential Cell Source for Treatment of ALS. American Society for Neural Transplantation and Repair (ASNTR). 2004.